Endoglin anticorps

N° du produit ABIN93943

Cet anticorps Souris Monoclonal détecte spécifiquement Endoglin dans WB, FACS, ICC et IHC (fro). Il présente une réactivité envers Humain et Porc et a été mentionné dans 1 publication.

Aperçu rapide pour Endoglin anticorps (ABIN93943)

Antigène: Endoglin (ENG)

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Endoglin est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MEM-229

Détail du produit anti-Endoglin anticorps

Fonction: Anti-Hu CD105 Purified

Specificité: The antibody MEM-229 recognizes an extracellular epitope of CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow, it is also present on syncytiotrophoblast on placenta throughout pregnancy.

Aucune reactivité croisée: Chien, Cheval

Réactivité croisée (Details): Human, Porcine

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 2-6 μg/mL, positive control: Kg1 human acute myelogenous leukemia cell line.
Immunohistochemistry (frozen sections): Recommended dilution: 1:200, acetone fixation.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Endoglin (ABIN93943)

Plánka, Necas, Srnec, Rauser, Starý, Jancár, Amler, Filová, Hlucilová, Kren, Gál: "Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs." dans: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 58, Issue 6, pp. 885-93, (2010) (PubMed).

Détails sur Endoglin

Antigène: Endoglin (ENG)

Autre désignation: CD105

Sujet: Endoglin,CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.,Endoglin, END, ENG, HHT1, ORW1

ID gène: 2022

UniProt: P17813