CD20 anticorps

N° du produit ABIN94027

Cet anticorps Souris Monoclonal détecte spécifiquement CD20 dans FACS. Il présente une réactivité envers Humain et a été mentionné dans 4+ publications.

Aperçu rapide pour CD20 anticorps (ABIN94027)

Antigène: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD20 est non-conjugé

Application: Flow Cytometry (FACS)

Clone: LT20

Détail du produit anti-CD20 anticorps

Fonction: Anti-Hu CD20 Purified

Specificité: The antibody LT20 reacts with an extracellular epitope of CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Normal human lymphocytes from lymph node.

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 3 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD20 (ABIN94027)

Všianská, Říhová, Varmužová, Suská, Kryukov, Mikulášová, Kupská, Penka, Pour, Adam, Hájek: "Analysis of B-cell subpopulations in monoclonal gammopathies." dans: Clinical lymphoma, myeloma & leukemia, Vol. 15, Issue 4, pp. e61-71, (2015) (PubMed).

Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." dans: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, (2007) (PubMed).

Teeling, Mackus, Wiegman, van den Brakel, Beers, French, van Meerten, Ebeling, Vink, Slootstra, Parren, Glennie, van de Winkel: "The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 362-71, (2006) (PubMed).

Chan, Hughes, French, Tutt, Walshe, Teeling, Glennie, Cragg: "CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts." dans: Cancer research, Vol. 63, Issue 17, pp. 5480-9, (2003) (PubMed).

Détails sur CD20

Antigène: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Autre désignation: CD20

Sujet: MS4A1,CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.,B1, S7, MS4A, Bp35, CVID5, LEU-16

ID gène: 931

UniProt: P11836