Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CD20 anticorps (APC)

MS4A1 Reactivité: Humain FACS Hôte: Souris Monoclonal LT20 APC
N° du produit ABIN94031
  • Antigène Voir toutes CD20 (MS4A1) Anticorps
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
    Reactivité
    • 278
    • 57
    • 23
    • 19
    • 18
    • 17
    • 11
    • 7
    • 6
    • 5
    • 2
    • 2
    Humain
    Hôte
    • 210
    • 96
    • 2
    • 2
    Souris
    Clonalité
    • 228
    • 80
    • 1
    Monoclonal
    Conjugué
    • 111
    • 21
    • 19
    • 17
    • 13
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CD20 est conjugé à/à la APC
    Application
    • 226
    • 93
    • 79
    • 48
    • 47
    • 38
    • 25
    • 22
    • 20
    • 17
    • 14
    • 13
    • 11
    • 11
    • 9
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificité
    The antibody LT20 reacts with an extracellular epitope of CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
    Réactivité croisée (Details)
    Human
    Purification
    Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogène
    Normal human lymphocytes from lymph node.
    Clone
    LT20
    Isotype
    IgG2a
    Top Product
    Discover our top product MS4A1 Anticorps primaire
  • Indications d'application
    Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
    Commentaires

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Reconstitution
    No reconstitution is necessary.
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Avoid prolonged exposure to light.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Všianská, Říhová, Varmužová, Suská, Kryukov, Mikulášová, Kupská, Penka, Pour, Adam, Hájek: "Analysis of B-cell subpopulations in monoclonal gammopathies." dans: Clinical lymphoma, myeloma & leukemia, Vol. 15, Issue 4, pp. e61-71, (2015) (PubMed).

    Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." dans: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, (2007) (PubMed).

    Teeling, Mackus, Wiegman, van den Brakel, Beers, French, van Meerten, Ebeling, Vink, Slootstra, Parren, Glennie, van de Winkel: "The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 362-71, (2006) (PubMed).

  • Antigène
    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
    Autre désignation
    CD20 (MS4A1 Produits)
    Synonymes
    anticorps B1, anticorps Bp35, anticorps CD20, anticorps CVID5, anticorps LEU-16, anticorps MS4A2, anticorps S7, anticorps AA960661, anticorps Cd20, anticorps Ly-44, anticorps Ms4a2, anticorps MS4A1, anticorps bp35, anticorps cd20, anticorps ms4a2, anticorps leu-16, anticorps ms4a4c, anticorps cd20-like, anticorps membrane spanning 4-domains A1, anticorps membrane-spanning 4-domains, subfamily A, member 1, anticorps B-lymphocyte antigen CD20, anticorps MS4A1, anticorps Ms4a1, anticorps ms4a1, anticorps LOC101694281
    Sujet
    MS4A1,CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.,B1, S7, MS4A, Bp35, CVID5, LEU-16
    ID gène
    931
    UniProt
    P11836
Vous êtes ici:
Support technique