CD53 anticorps

N° du produit ABIN94171

Cet anticorps Souris Monoclonal détecte spécifiquement CD53 dans FACS, WB, IP et IHC (fro). Il présente une réactivité envers Humain et a été mentionné dans 4+ publications.

Aperçu rapide pour CD53 anticorps (ABIN94171)

Antigène: CD53

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD53 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MEM-53

Détail du produit anti-CD53 anticorps

Fonction: Anti-Hu CD53 Purified

Specificité: The antibody MEM-53 reacts with an extracellular epitope of CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes, it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Leukocytes of pacient suffering from a LGL-type leukemia.

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry (frozen sections): It is suitable for discrimination of lymphomas from other tumors.
Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD53 (ABIN94171)

Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." dans: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Szöllósi, Horejsí, Bene, Angelisová, Damjanovich: "Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2939-46, (1996) (PubMed).

Olweus, Lund-Johansen, Terstappen: "CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells." dans: Blood, Vol. 85, Issue 9, pp. 2402-13, (1995) (PubMed).

Rasmussen, Blomhoff, Stokke, Horejsi, Smeland: "Cross-linking of CD53 promotes activation of resting human B lymphocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 4997-5007, (1994) (PubMed).

Détails sur CD53

Antigène: CD53

Autre désignation: CD53

Sujet: CD53 Molecule,CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.,Tetraspanin-25, MOX44, TSPAN25

ID gène: 963

UniProt: P19397