ICAM1 anticorps

N° du produit ABIN94178

L’anticorps Souris Monoclonal anti-ICAM1 a été validé pour WB, FACS, ICC, IHC (p) et IHC (fro). Il convient pour détecter ICAM1 dans des échantillons de Humain.

Aperçu rapide pour ICAM1 anticorps (ABIN94178)

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ICAM1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1H4

Détail du produit anti-ICAM1 anticorps

Fonction: Anti-Hu CD54 Purified Azide Free

Specificité: The antibody 1H4 recognizes an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells, the expression of CD54 is upregulated by activation.

Réactivité croisée (Details): Other not tested, Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Raji cells and spleen cells fused with NS1 cells

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry (frozen sections): Recommended dilution: 5-10 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required.
Flow cytometry: Recommended dilution: 1-4 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, non-reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Agent conservateur: Azide free

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur ICAM1

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: CD54

Sujet: Intercellular adhesion molecule 1,CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.,ICAM-1, BB2, P3.58

ID gène: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling