CD97 anticorps (CD97) (FITC)

Details for Product anti-CD97 Antibody No. ABIN94253, Fournisseur: Connectez-vous pour afficher
Antigène
  • AA409984
  • TM7LN1
  • adhesion G protein-coupled receptor E5
  • Adgre5
  • ADGRE5
Reactivité
Humain
166
65
24
5
2
1
Hôte
Souris
108
69
17
14
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp CD97 est conjugé à/à la FITC
17
14
11
7
5
4
3
3
3
3
3
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
107
74
62
53
25
25
13
9
9
5
4
4
2
2
2
1
1
1
1
Options
Fournisseur
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N° du produit (Fournisseur)
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Immunogène PHA-activated peripheral blood cells
Clone MEM-180
Isotype IgG1
Specificité The antibody MEM-180 recognizes a unique epitope on CD97, a 75-85 kDa surface glycoprotein of G-protein-coupled receptor family, expressed on activated B and T lymphocytes, monocytes/macrophages, dendritic cells and granulocytes.
HLDA VI, WS Code BP 415
HLDA VI, WS Code NL N-L023
Réactivité croisée (Details) Human
Antigène
Autre désignation CD97 (CD97 Antibody Extrait)
Sujet CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
Indications d'application The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Commentaires

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Do not freeze.
Avoid prolonged exposure to light.
Stock 4 °C
Stockage commentaire Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Images (Fournisseur)
Flow Cytometry (FACS) image for anti-CD97 (CD97) antibody (FITC) (ABIN94253) Surface staining of human peripheral blood with anti-CD97 (MEM-180) FITC.
Flow Cytometry (FACS) image for anti-CD97 (CD97) antibody (FITC) (ABIN94253) Surface staining of human peripheral blood with anti-CD97 (MEM-180) FITC.
Background publications Wang, Tian, Haino, Gao, Lake, Ward, Wang, Siebenlist, Murphy, Kelly: "Improved antibacterial host defense and altered peripheral granulocyte homeostasis in mice lacking the adhesion class G protein receptor CD97." dans: Infection and immunity, Vol. 75, Issue 3, pp. 1144-53, 2007 (PubMed).

Abbott, Spendlove, Roversi, Fitzgibbon, Knott, Teriete, McDonnell, Handford, Lea: "Structural and functional characterization of a novel T cell receptor co-regulatory protein complex, CD97-CD55." dans: The Journal of biological chemistry, Vol. 282, Issue 30, pp. 22023-32, 2007 (PubMed).

Galle, Sittig, Hanisch, Wobus, Wandel, Loeffler, Aust: "Individual cell-based models of tumor-environment interactions: Multiple effects of CD97 on tumor invasion." dans: The American journal of pathology, Vol. 169, Issue 5, pp. 1802-11, 2006 (PubMed).

Visser, de Vos, Hamann, Melief, van Meurs, van Lier, Laman, Hintzen: "Expression of the EGF-TM7 receptor CD97 and its ligand CD55 (DAF) in multiple sclerosis." dans: Journal of neuroimmunology, Vol. 132, Issue 1-2, pp. 156-63, 2002 (PubMed).

Produit citée dans: Wobus, Vogel, Schmücking, Hamann, Aust: "N-glycosylation of CD97 within the EGF domains is crucial for epitope accessibility in normal and malignant cells as well as CD55 ligand binding." dans: International journal of cancer. Journal international du cancer, Vol. 112, Issue 5, pp. 815-22, 2004 (PubMed).

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