Cet anticorps Souris Monoclonal détecte spécifiquement C-Peptide dans ELISA, ICC, IHC (p) et RIA. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.
The mouse monoclonal antibody C-PEP-01 reacts specifically with C-peptide, a part of the proinsulin molecule. Proinsulin consists of three parts: C-peptide and two long strands of amino acids (alpha and beta chains, later become linked together to form the insulin molecule). No cross-reactivity with insulin or other peptide hormones or proteins was observed.
Réactivité croisée (Details)
Human
Purification
Purified by protein-A affinity chromatography.
Pureté
> 95 % (by SDS-PAGE)
Immunogène
Human C-peptide conjugated to bovine serum albumin.
Reactivité: Porc
WB, IHC, IP, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Immunohistochemistry (paraffin sections): Recommended dilution: 2-5 μg/mL, positive control: human pancreas (islets of Langerhans).
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze.
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
Koblas, Zacharovova, Berkova, Girman, Saudek: "An acidic pH and activation of phosphoinositide 3-kinase stimulate differentiation of pancreatic progenitors into insulin-producing cells." dans: Transplantation proceedings, Vol. 42, Issue 6, pp. 2075-80, (2010) (PubMed).
Hilgert, Stolba, Kristofová, Stefanová, Bendlová, Lebl, Horejsí: "A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA." dans: Hybridoma, Vol. 10, Issue 3, pp. 379-86, (1991) (PubMed).
Antigène
C-Peptide
Sujet
From every molecule of proinsulin, one molecule of insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to insulin.,C-peptide of proinsulin