Cytokeratins anticorps

N° du produit ABIN94275

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans ICFC, ICC, IHC (p), IP et WB. Adapté pour Mammifères. Ce Primary Antibody a été cité dans 6+ publications.

Aperçu rapide pour Cytokeratins anticorps (ABIN94275)

Antigène: Cytokeratins

Reactivité: Mammifères

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Clone: C-11

Détail du produit

Fonction: Anti-Cytokeratins Purified

Specificité: The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.

Réactivité croisée (Details): Mammalian

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.
Immunohistochemistry: Recommended dilution: 2-8 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Haun, Devaraj, Marinelli, Lee, Weissleder: "Probing intracellular biomarkers and mediators of cell activation using nanosensors and bioorthogonal chemistry." dans: ACS nano, Vol. 5, Issue 4, pp. 3204-13, (2011) (PubMed).

Bianchi, Bombelli, Raimondo, Torsello, Angeloni, Ferrero, Di Stefano, Chinello, Cifola, Invernizzi, Brambilla, Magni, Pitto, Zanetti, Mocarelli, Perego: "Primary cell cultures from human renal cortex and renal-cell carcinoma evidence a differential expression of two spliced isoforms of Annexin A3." dans: The American journal of pathology, Vol. 176, Issue 4, pp. 1660-70, (2010) (PubMed).

Broekema, Harmsen, Koerts, Petersen, van Luyn, Navis, Popa: "Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats." dans: Kidney international, Vol. 68, Issue 6, pp. 2572-81, (2005) (PubMed).

Hamakawa, Sumida, Tanioka, Sogawa, Yamada: "Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis." dans: Research communications in molecular pathology and pharmacology, Vol. 101, Issue 2, pp. 115-26, (1999) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." dans: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." dans: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).

Détail du antigène

Antigène: Cytokeratins

Sujet: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,cytokeratin, CYK, CK, KRT