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Cytokeratin 19 anticorps

Cet anticorps anti-Cytokeratin 19 est un anticorps Souris Monoclonal détectant Cytokeratin 19 dans WB, ELISA, IHC (p), ICC, IP et ICFC. Adapté pour Humain. Ce Primary Antibody a été cité dans 2+ publications.
N° du produit ABIN94290

Aperçu rapide pour Cytokeratin 19 anticorps (ABIN94290)

Antigène

Voir toutes Cytokeratin 19 (KRT19) Anticorps
Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivité

  • 309
  • 114
  • 85
  • 8
  • 7
  • 6
  • 5
  • 5
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Humain

Hôte

  • 162
  • 158
  • 3
  • 2
  • 1
Souris

Clonalité

  • 187
  • 139
Monoclonal

Conjugué

  • 139
  • 37
  • 19
  • 12
  • 9
  • 7
  • 7
  • 7
  • 7
  • 7
  • 7
  • 6
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Cet anticorp Cytokeratin 19 est non-conjugé

Application

  • 235
  • 131
  • 107
  • 81
  • 79
  • 45
  • 43
  • 41
  • 39
  • 33
  • 32
  • 32
  • 21
  • 7
  • 6
  • 5
  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC)

Clone

A53-B-A2
  • Fonction

    Anti-Cytokeratin 19 Purified

    Specificité

    The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.

    Réactivité croisée (Details)

    Human

    Purification

    Purified by protein-A affinity chromatography.

    Pureté

    > 95 % (by SDS-PAGE)

    Immunogène

    MCF-7 human breast adenocarcinoma cell line

    Isotype

    IgG2a
  • Indications d'application

    Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
    Immunohistochemistry: Recommended dilution: 5-10 μg/mL.
    Western blotting: Recommended dilution: 1-2 μg/mL, positive control: MCF-7, BA-17, HT-29 cells.

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Do not freeze.

    Stock

    4 °C

    Stockage commentaire

    Store at 2-8°C. Do not freeze.
  • Kasper, Moll, Stosiek, Karsten: "Patterns of cytokeratin and vimentin expression in the human eye." dans: Histochemistry, Vol. 89, Issue 4, pp. 369-77, (1988) (PubMed).

    Karsten, Papsdorf, Roloff, Stolley, Abel, Walther, Weiss: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." dans: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, (1985) (PubMed).

  • Antigène

    Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

    Autre désignation

    Cytokeratin 19

    Sujet

    Keratin 19,Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K19, CK19, CYK19, KRT19, K1CS

    ID gène

    3880

    UniProt

    P08727
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