Cité dans 1 publication.
Cet anticorps anti-Keratin 7/17 Monoclonal Souris (Clone C-46) (ABIN94296) détecte spécifiquement Keratin 7/17 dans WB, IP, ICC et IHC (p).
L’anticorps est réactif avec des échantillons de Humain, Boeuf (Vache) et Porc.
The antibody C-46 reacts with cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.
Purification
Purified by protein-A affinity chromatography.
Pureté
> 95 % (by SDS-PAGE)
Immunogène
Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, pretreatment: 0,1 % pepsin, 30 min, 37 °C, incubation 4 °C overnight, chromogen DAB, positive tissue: pancreas. Western blotting: Recommended dilution: 1-2 μg/mL, positive control: HeLa cells.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
Vojt?sek, Stasková, Nenutil, Bártková, Kovarík, Rejthar, Bártek: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." dans: Neoplasma, Vol. 37, Issue 3, pp. 333-42, (1990) (PubMed).
Antigène
Keratin 7/17 (KRT7/17)
Autre désignation
Cytokeratin 7/17
Sujet
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K7, K17, CK7, CD17, CK17, CYK7, CYK17, KRT7, KRT17, SCL, K2C7