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IgE anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement IgE dans ELISA, WB et FACS. Il présente une réactivité envers Humain.
N° du produit ABIN94399

Aperçu rapide pour IgE anticorps (ABIN94399)

Antigène

Voir toutes IgE Anticorps
IgE

Reactivité

  • 23
  • 3
  • 2
  • 1
Humain

Hôte

  • 16
  • 10
  • 2
  • 1
Souris

Clonalité

  • 21
  • 7
  • 1
Monoclonal

Conjugué

  • 21
  • 3
  • 2
  • 1
  • 1
  • 1
Cet anticorp IgE est non-conjugé

Application

  • 18
  • 9
  • 8
  • 5
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Clone

4H10
  • Fonction

    Anti-Hu IgE Purified

    Specificité

    The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7.325 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).

    Réactivité croisée (Details)

    Human

    Purification

    Purified by protein-A affinity chromatography.

    Pureté

    > 95 % (by SDS-PAGE)

    Immunogène

    Purified human IgE.

    Isotype

    IgG1
  • Indications d'application

    Flow cytometry: Recommended dilution: 4 μg/mL.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Do not freeze.

    Stock

    4 °C

    Stockage commentaire

    Store at 2-8°C. Do not freeze.
  • Antigène

    IgE

    Sujet

    Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E

    Poids moléculaire

    180 kDa
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