Ig Light Chains anticorps

N° du produit ABIN94401

Cité dans 1 publication. Cet anticorps anti-Ig Light Chains Monoclonal Souris (Clone IVA285-1) (ABIN94401) détecte spécifiquement Ig Light Chains dans FACS, IHC (fro) et WB. L’anticorps est réactif avec des échantillons de Boeuf (Vache) et Mouton.

Aperçu rapide pour Ig Light Chains anticorps (ABIN94401)

Antigène: Ig Light Chains

Reactivité: Boeuf (Vache), Mouton

Hôte: Souris

Clonalité: Monoclonal

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Clone: IVA285-1

Détail du produit

Fonction: Anti-Bov Ig Light Chains Purified

Réactivité croisée (Details): Bovine, Sheep

Attributs du produit: The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Fraction of Bovine immunoglobulin.

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 0,5-1 μg/mL, overnight at 4 °C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12 % separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." dans: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).

Détail du antigène

Antigène: Ig Light Chains

Sujet: Immunoglobulin light chains

ID gène: 35