The antibody KN-03 recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments The antibody KN-03 well recognizes kinesin bound to taxol-stabilized microtubules.
Réactivité croisée (Details)
Mouse, Human, Rat, Other not tested, Porcine
Purification
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze.
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
Mac?rek, Dráberová, Richterová, Böhm, Dráber: "Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin." dans: Hybridoma and hybridomics, Vol. 21, Issue 6, pp. 457-62, (2003) (PubMed).
Antigène
Kinesin (heavy chain)
Synonymes
anticorps KINH, anticorps KNS, anticorps KNS1, anticorps UKHC, anticorps kinesin family member 5B, anticorps kinesin family member 5C, anticorps KIF5B, anticorps Kif5c
Sujet
Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.,KHC, KINHC, KIF5