NEFM anticorps

N° du produit ABIN94435

Cet anticorps Souris Monoclonal détecte spécifiquement NEFM dans WB, ICC et IHC (p). Il présente une réactivité envers Mammifères et a été mentionné dans 1 publication.

Aperçu rapide pour NEFM anticorps (ABIN94435)

Antigène: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivité: Mammifères

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFM est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NF-09

Détail du produit anti-NEFM anticorps

Fonction: Anti-Neurofilament M Purified

Specificité: The antibody NF-09 reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa intracellular antigen) of various species.

Réactivité croisée (Details): Mammalian

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Pellet of porcine brain cold stable proteins after depolymerization of microtubules.

Isotype: IgG2a

Information d'application

Indications d'application: Immunocytochemistry: Positive tissue: Neuro2A murine cell line, Carnoys fixative 2 x 3 min, blocking 1 % glycine + 0.2 % gelatin 10 min.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-NEFM (ABIN94435)

Dráberová, Sulimenko, Kukharskyy, Dráber: "Monoclonal antibody NF-09 specific for neurofilament protein NF-M." dans: Folia biologica, Vol. 45, Issue 4, pp. 163-5, (2000) (PubMed).

Détails sur NEFM

Antigène: NEFM (Neurofilament Triplet M Protein (NEFM))

Autre désignation: Neurofilament M

Sujet: Neurofilament medium,Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights –, neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.,NFM, NEFM, Neurofilament medium chain, NEF3

ID gène: 4741

UniProt: P07197

Pathways: Brown Fat Cell Differentiation