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Kinesin anticorps (AA 331-906)

Reactivité: Humain, Souris, Porc WB, ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN94498
  • Antigène Tous les produits Kinesin
    Kinesin
    Épitope
    • 16
    • 2
    • 1
    • 1
    • 1
    AA 331-906
    Reactivité
    • 21
    • 19
    • 4
    • 3
    • 2
    Humain, Souris, Porc
    Hôte
    • 19
    • 6
    Lapin
    Clonalité
    • 19
    • 6
    Polyclonal
    Conjugué
    • 10
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Kinesin est non-conjugé
    Application
    • 14
    • 13
    • 13
    • 8
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunocytochemistry (ICC)
    Specificité
    The polyclonal antibody detects total level of endogenous kinesin protein (intracellular antigen).
    Réactivité croisée (Details)
    Human, Mouse, Porcine, Other not tested
    Purification
    Purified by ligand affinity chromatography.
    Pureté
    > 95 % (by SDS-PAGE)
    Immunogène
    stalk domain of human kinesin (aa 331-906) expressed in E. coli (FKHC3)
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Do not freeze.
  • Malcová-Janatová, Richterová, Dráber, Hasek: "Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains." dans: Folia microbiologica, Vol. 49, Issue 6, pp. 665-70, (2005) (PubMed).

  • Antigène
    Kinesin
    Abstract
    Kinesin Produits
    Synonymes
    anticorps kinesin, anticorps putative kinesin, anticorps Tc00.1047053510323.120, anticorps Tc00.1047053508153.200, anticorps LmjF29.2390, anticorps LINJ_29_2500, anticorps LBRM_29_2360
    Sujet
    Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.,KIN
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