Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Réactivité croisée (Details)
Cross reactivity against Acid Phosphatase from other tissues and species may occur but have not been specifically determined.
Pureté
Anti-ACID PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].
niveau d'endotoxine
Low Endotoxin : No
Immunogène
Acid Phosphatase [Potato] Immunogen Type: Native Protein
ACP
Reactivité: Souris
WB, IF (cc), IF (p)
Hôte: Lapin
Polyclonal
Alexa Fluor 555
Indications d'application
ELISA Dilution: 1:4.000 - 1:40.000 Immunohistochemistry Dilution: 1:1.000 - 1:5.000 Western Blot Dilution: 1:2.000 - 1:10.000
Restrictions
For Research Use only
Format
Liquid
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Conseil sur la manipulation
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Date de péremption
Expiration date is one (1) year from date of opening.