ATP1A1 anticorps

N° du produit ABIN94710

N° du produit (Fournisseur): 200-9155

Cet anticorps anti-ATP1A1 est un anticorps Poulet Polyclonal détectant ATP1A1 dans WB, ELISA et IP. Adapté pour Chien.

Aperçu rapide pour ATP1A1 anticorps (ABIN94710)

Antigène: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivité: Chien

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp ATP1A1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-ATP1A1 anticorps

N° du produit (Fournisseur): 200-9155

Fournisseur: Rockland

Fonction: ATPase Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney].

Attributs du produit: Synonyms: chicken anti-ATPase Antibody, Sodium/potassium-transporting ATPase

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: ATPase, Sodium, Potassium [Canine Kidney]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-ATPase Na+ K+ has been assayed against 1.0 μg of ATPase, Sodium, Potassium in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit) code #603-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur ATP1A1

Antigène: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Autre désignation: ATP1A1

Sujet: Background: Sodium/potassium-transporting ATPase subunit alpha-1 is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

ID gène: 403992

NCBI Accession: NP_001003306

UniProt: P50997

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process