His Tag anticorps
Aperçu rapide pour His Tag anticorps (ABIN950190)
Antigène
Voir toutes His Tag AnticorpsHôte
Clonalité
Conjugué
Application
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Séquence
- HHHHHH
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Purification
- Purified
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Immunogène
- 6X-Histidine peptide conjugated to Keyhole Limpet Hemocyanin protein and emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. To make up the final mixture, the IgY fraction was spiked with affinity purified antibody, mixed with Glycerol and Thimerosal, and filter-sterilized.
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Isotype
- Ig Fraction
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 10 mg/mL (based on absorbance at 280 nm)
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Buffer
- Sodium Phosphate-Buffered (10 mM) isotonic (0.9 % , w/v) Saline (PBS, pH 7.2) (50 % by volume) with 50 % Glycerol as an anti-freezing agent and 0.01 % Thimerosal as preservative.
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Agent conservateur
- Thimerosal (Merthiolate)
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Précaution d'utilisation
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- His Tag
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Autre désignation
- 6xHistidine Epitope Tag
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Classe de substances
- Tag
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Sujet
- In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
Antigène
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