ACPP anticorps
Aperçu rapide pour ACPP anticorps (ABIN950249)
Antigène
Voir toutes ACPP AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Recognizes Mouse Prostatic Acid Phosphatase (PAP).
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Purification
- Affinity Chromatography using a peptide column.
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Immunogène
- Recombinant Mouse PAP protein was expressed using a baculoviral-delivery system. Preparation: After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (“anti-PAP IgY fraction”). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (“Affinity-purified anti-PAP”). The final preparation in the accompanying vial contains 10 mg/ml of the “anti-PAP IgY fraction” supplemented with 20 mg/ml of the “affinity-purified anti-PAP” plus 50% (v/v) Glycerol (to prevent freezing at –20°C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 µl aliquots prepared.
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Isotype
- Ig Fraction
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 10 mg IgY/ml (based on absorbance at 280 nm)
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Buffer
- 10 mM PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Store the antibody undiluted in the dark at 2-8 °C.
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- ACPP (Acid Phosphatase, Prostate (ACPP))
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Autre désignation
- ACPP / Prostatic Acid Phosphatase
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Sujet
- Mouse PAP is a 43,698 dalton protein (381 amino acids, NCBI accession numberAAF23171) associated with prostatic cancer cells, as well as primary afferent sensory neurons involved in the pain pathway. This protein is an enzyme that dephosphorylates adenosine monophosphate (AMP) in the dorsal horn gray matter of the spinal cord, generating free adenosine. Injections of PAP into the dorsal horn of experimental mice has been shown to decrease pain perception by acting in an antinociceptive, antihyperalgesic, and antiallodynic fashion.Synonyms: ACP3, PAP, PSAP, Prostate acid phosphatase
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ID gène
- 56318
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NCBI Accession
- NP_062781
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Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
Antigène
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