GLB1 anticorps

N° du produit ABIN950653

Cet anticorps Poulet Polyclonal détecte spécifiquement GLB1 dans WB, IF et IHC (fro). Il présente une réactivité envers E. coli.

Aperçu rapide pour GLB1 anticorps (ABIN950653)

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivité: E. coli

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp GLB1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-GLB1 anticorps

Specificité: This antibody recognizes the LacZ gene product with high titre and high specificity. This anitbody can be used together with Rabbit and Mouse primaty antibodies for Double Immunostaining with no Secondary antibody cross reactivity. It even works well with fusion protein of LacZ! Quality Control: This antibody preparation was analyzed by Immunohistochemistry (at a dilution of 1/5000) using tissues infected with a virus bearing the LacZ gene. Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FC-N, 1/2000 dilution) was used as a secondary antibody.

Purification: Purified

Immunogène: Purified Beta-Galactosidase (LacZ) protein emulsified in Freund's adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using Beta-Galactosidase conjugated to an Agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 myg/ml) and purified IgY (10 mg/ml), and is filter-sterilized.

Isotype: Ig Fraction

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10.0 mg/mL (based on absorbance at 280 nm)

Buffer: Sodium Phosphate-Buffered (10 mM) isotonic (0.9 % , w/v) Saline (PBS, pH 7.2) (50 % by volume) with 50 % Glycerol as an anti-freezing agent and 0.01 % Thimerosal as preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial before opening.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C in the dark.

Détails sur GLB1

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Autre désignation: beta Galactosidase (GAL)

Sujet: Beta-galactosidase (beta-gal), an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 114 kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.Synonyms: Beta-Gal Fusion Protein, Beta-Gal tag, JW0335, Lactase, b0344, lacZ tag

ID gène: 945006

NCBI Accession: AP_000996

Pathways: Glycosaminoglycan Metabolic Process