BMP7 anticorps (AA 316-431)

N° du produit ABIN950669

Cet anticorps Lapin Polyclonal détecte spécifiquement BMP7 dans WB. Il présente une réactivité envers Humain.

Aperçu rapide pour BMP7 anticorps (AA 316-431) (ABIN950669)

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BMP7 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-BMP7 anticorps

Épitope: AA 316-431

Specificité: Recognizes Human BMP7.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Protein A Chromatography

Immunogène: Highly pure (>95%) recombinant Human BMP-7 (Ala316-His431) derived from E. coli.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable at RT for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C. Avoid repeated freeze-thaw cycles!

Date de péremption: 6 months

Détails sur BMP7

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Autre désignation: BMP7

Sujet: Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP1), is a widely expressed TGFβ superfamily member with important functions during embryogenesis, in the adult, and in disease (1,2). Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain (3). The growth factor domain of human BMP-7 shares 98 % aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but often remains associated with the mature C-terminus. Based on in vivo and in vitro studies, BMP-7 has the potential to be secreted as a disulfide-linked mature homodimer, or particularly as a heteromeric complex that consists of two propeptides noncovalently associated with a mature disulfide linked homodimer (5,6). The presence of the propeptides in BMP-7 appears to stabilize the molecule and provide a docking mechanism for extracellular storage on molecules such as fibrillin1 and 2 (5,6). The propeptides themselves do not impart latency to the complex. BMP-7 binding to type II receptors rapidly displaces the prodomain: mature molecule interaction and has no effect on activity. But it is suggested that immobilized BMP-7 (via prodomain:fibrillin) is inactive, allowing for possible long term storage of the molecule (6). BMP-7 interacts with the type 2 receptors Activin RIIA, Activin RIIB, and BMPRII and the type 1 receptors Activin RIA, BMPRIA, and BMPRIB (2,6). BMP-7 may also be processed into a disulfide-linked heterodimer with either BMP-2 or BMP-4. Such complexes may show increased potency and range of activity compared to BMP7 homodimers (7-9). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (10,11), inhibits the branching of prostate epithelium (12), and antagonizes epithelial mesenchymal transition (EMT) (13-15). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (14), ameliorates fibrotic damage in nephritis (13), and promotes neuro regeneration following brain ischemia (16).Synonyms: BMP-7, Bone morphogenetic protein 7, OP1, Osteogenic protein 1

ID gène: 655

NCBI Accession: NP_001710

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance