Bilirubin Oxidase anticorps (Biotin)

N° du produit ABIN95598

N° du produit (Fournisseur): 200-106-212-0100

L’anticorps Chèvre Polyclonal anti-Bilirubin Oxidase a été validé pour WB, IP et ELISA. Il convient pour détecter Bilirubin Oxidase dans des échantillons de Myrothecium verrucaria.

Aperçu rapide pour Bilirubin Oxidase anticorps (Biotin) (ABIN95598)

Antigène: Bilirubin Oxidase

Reactivité: Myrothecium verrucaria

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Bilirubin Oxidase est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Détail du produit anti-Bilirubin Oxidase anticorps (Biotin)

N° du produit (Fournisseur): 200-106-212-0100

Fournisseur: Rockland

Fonction: Bilirubin Oxidase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria].

Attributs du produit: Synonyms: goat anti-Bilirubin Oxidase biotin conjugation, goat anti-Bilirubin Oxidase Antibody biotin Conjugated

Purification: Anti-Bilirubin Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Bilirubin Oxidase [Myrothecium verrucaria]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Bilirubin Oxidase has been tested by western blot and is suitable to be assayed against 1.0 μg of Bilirubin Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Bilirubin Oxidase

Antigène: Bilirubin Oxidase

Sujet: Background: Anti-Bilirubin Oxidase antibody is an enzyme that increases the oxidation rate of bilirubin to biliverdin and other substrates. It is ideal for investigators interested in Metabolism or Signal Transduction research.

UniProt: Q12737