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Biotin anticorps

L’anticorps Chèvre Polyclonal anti-Biotin a été validé pour WB, ELISA et IHC. Il convient pour détecter Biotin dans des échantillons de .
Rockland
N° du produit ABIN95606
N° du produit (Fournisseur): 100-1198

Aperçu rapide pour Biotin anticorps (ABIN95606)

Antigène

Biotin

Reactivité

Veuillez nous consulter SVP

Hôte

  • 63
  • 42
  • 38
Chèvre

Clonalité

  • 82
  • 53
  • 1
Polyclonal

Conjugué

  • 36
  • 14
  • 9
  • 8
  • 8
  • 6
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
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  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Biotin est non-conjugé

Application

  • 77
  • 56
  • 56
  • 51
  • 39
  • 33
  • 16
  • 13
  • 11
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • N° du produit (Fournisseur)

    100-1198

    Fournisseur

    Rockland

    Fonction

    Biotin Antibody

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, biotinylated IgG and Biotinylated Bovine Serum Albumin (BSA).

    Attributs du produit

    Synonyms: Goat anti-Biotin Antibody, Goat anti Biotin

    Purification

    This product was prepared from monospecific antiserum by a delipidation and defibrination.

    Immunogène

    Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

    Immunogen Type: Native Protein

  • Indications d'application

    Immunohistochemistry Dilution: User Optimized

    Application Note: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

    Western Blot Dilution: 1:10,000 - 1:50,000

    ELISA Dilution: 1:100,000 - 1:500,000

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration

    85 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Date de péremption

    12 months
  • Antigène

    Biotin

    Classe de substances

    Chemical

    Sujet

    Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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