Biotin anticorps (HRP)

N° du produit ABIN95620

N° du produit (Fournisseur): 600-103-098

Cet anticorps Chèvre Polyclonal détecte spécifiquement Biotin dans WB, ELISA, IHC et DB. Il présente une réactivité envers .

Aperçu rapide pour Biotin anticorps (HRP) (ABIN95620)

Antigène: Biotin

Reactivité: Veuillez nous consulter SVP

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Biotin est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Détail du produit anti-Biotin anticorps (HRP)

N° du produit (Fournisseur): 600-103-098

Fournisseur: Rockland

Fonction: Biotin Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, biotinylated bovine serum albumin and Biotinylated IgG.

Attributs du produit: Synonyms: goat anti-Biotin Antibody HRP Conjugation, Peroxidase conjugated goat anti-Biotin Antibody

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.

Immunogène:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Other

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Biotin Peroxidase conjugated antibody has been tested by ELISA and dot blot. This product is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:3,000 - 1:15,000

ELISA Dilution: 1:140,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Peroxidase Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.