Biotin anticorps

N° du produit ABIN95626

N° du produit (Fournisseur): 100-4198

Cet anticorps Lapin Polyclonal détecte spécifiquement Biotin dans WB, ELISA et IHC. Il présente une réactivité envers et a été mentionné dans 8+ publications.

Aperçu rapide pour Biotin anticorps (ABIN95626)

Antigène: Biotin

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Biotin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-Biotin anticorps

N° du produit (Fournisseur): 100-4198

Fournisseur: Rockland

Fonction: Biotin Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, biotinylated IgG and Biotinylated BSA.

Attributs du produit: Synonyms: rabbit anti-biotin antibody, rabbit anti biotin

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Native Protein

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Biotin has been tested in ELISA and is suitable for immunoblotting (western or dot blot), immunoprecipitation and most immunological methods requiring high titer and specificity.

Western Blot Dilution: 1:10,000 - 1:25,000

ELISA Dilution: 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 78 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Références pour anticorps anti-Biotin (ABIN95626)

Jongsma, Bakker, Cabukusta, Liv, van Elsland, Fermie, Akkermans, Kuijl, van der Zanden, Janssen, Hoogzaad, van der Kant, Wijdeven, Klumperman, Berlin, Neefjes: "SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport." dans: The EMBO journal, Vol. 39, Issue 6, pp. e102301, (2020) (PubMed).

Landi, Mari, Kleiser, Wolf, Gretzmeier, Wilhelm, Kiritsi, Thünauer, Geiger, Nyström, Reggiori, Claudinon, Römer: "Pseudomonas aeruginosa lectin LecB impairs keratinocyte fitness by abrogating growth factor signalling." dans: Life science alliance, Vol. 2, Issue 6, (2020) (PubMed).

Koerver, Papadopoulos, Liu, Kravic, Rota, Brecht, Veenendaal, Polajnar, Bluemke, Ehrmann, Klumperman, Jäättelä, Behrends, Meyer: "The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage." dans: EMBO reports, Vol. 20, Issue 10, pp. e48014, (2019) (PubMed).

Sztal, Zhao, Williams, Oorschot, Parslow, Giousoh, Yuen, Hall, Costin, Ramm, Bird, Busch-Nentwich, Stemple, Currie, Cooper, Laing, Nowak, Bryson-Richardson: "Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function." dans: Acta neuropathologica, Vol. 130, Issue 3, pp. 389-406, (2016) (PubMed).

Wolf, Kastner, Lührmann: "Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex." dans: RNA (New York, N.Y.), Vol. 18, Issue 7, pp. 1347-57, (2012) (PubMed).

Jubelin, Taieb, Duda, Hsu, Samba-Louaka, Nobe, Penary, Watrin, Nougayrède, Schulman, Stebbins, Oswald: "Pathogenic bacteria target NEDD8-conjugated cullins to hijack host-cell signaling pathways." dans: PLoS pathogens, Vol. 6, Issue 9, pp. e1001128, (2011) (PubMed).

Duda, Borg, Scott, Hunt, Hammel, Schulman: "Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation." dans: Cell, Vol. 134, Issue 6, pp. 995-1006, (2008) (PubMed).

Peden, Oorschot, Hesser, Austin, Scheller, Klumperman: "Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins." dans: The Journal of cell biology, Vol. 164, Issue 7, pp. 1065-76, (2004) (PubMed).

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.