MANF anticorps

N° du produit ABIN956417

Détail du produit anti-MANF anticorps

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MANF est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Human MANF

Réactivité croisée (Details): No cross-reactivity in MANF-knock out mouse and with CDNF in IHC, slightly cross-reacts with purified CDNF in WB

Purification: MANF-affinity purified

Immunogène: Recombinant human MANF protein produced using CHO-basedIcosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purifiedfrom cell culture supernatant

Isotype: IgG

Information d'application

Indications d'application: ELISA1:200 000, Western immunoblotting 1:2000 – 1:6000, IHC, IF.  Polyclonal antibody working titer has to be established practically for each particularantigen and assay format

Commentaires:

Synonym: ARMET, ARP

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.45 mg/ml

Buffer: Concentrated ammonium sulphide in PBS pH 7.4.

Conseil sur la manipulation: As product is (NH4)2SO4precipitate, mix well by pipetting or vortexing prior use.

Stock: 4 °C

Stockage commentaire: This product is shipped in non-frozen liquid form in ambient conditions

Références pour anticorps anti-MANF (ABIN956417)

Herranen, Ikäheimo, Lankinen, Pakarinen, Fritzsch, Saarma, Lindahl, Pirvola: "Deficiency of the ER-stress-regulator MANF triggers progressive outer hair cell death and hearing loss." dans: Cell death & disease, Vol. 11, Issue 2, pp. 100, (2020) (PubMed).

Lindahl, Chalazonitis, Palm, Pakarinen, Danilova, Pham, Setlik, Rao, Võikar, Huotari, Kopra, Andressoo, Piepponen, Airavaara, Panhelainen, Gershon, Saarma: "Cerebral dopamine neurotrophic factor-deficiency leads to degeneration of enteric neurons and altered brain dopamine neuronal function in mice." dans: Neurobiology of disease, Vol. 134, pp. 104696, (2020) (PubMed).

Pakarinen, Danilova, Võikar, Chmielarz, Piepponen, Airavaara, Saarma, Lindahl: "MANF Ablation Causes Prolonged Activation of the UPR without Neurodegeneration in the Mouse Midbrain Dopamine System." dans: eNeuro, Vol. 7, Issue 1, (2020) (PubMed).

Tseng, Danilova, Domanskyi, Saarma, Lindahl, Airavaara: "MANF Is Essential for Neurite Extension and Neuronal Migration in the Developing Cortex." dans: eNeuro, Vol. 4, Issue 5, (2018) (PubMed).

Détails sur MANF

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Autre désignation: MANF (MANF Produits)

Synonymes: anticorps ARMET, anticorps ARP, anticorps 3230402M22Rik, anticorps AA407711, anticorps AA408789, anticorps AA673178, anticorps Armet, anticorps D17914, anticorps D18Mgi17, anticorps MANF, anticorps RBM15B, anticorps ARP-like, anticorps CG7013, anticorps DmMANF, anticorps Dmel\\CG7013, anticorps anon-EST:ParkEST188, anticorps arp, anticorps armet, anticorps im:6909481, anticorps zgc:153327, anticorps manf, anticorps mesencephalic astrocyte derived neurotrophic factor, anticorps mesencephalic astrocyte-derived neurotrophic factor, anticorps RNA binding motif protein 15B, anticorps Mesencephalic astrocyte-derived neurotrophic factor, anticorps mesencephalic astrocyte-derived neurotrophic factor S homeolog, anticorps MANF, anticorps Manf, anticorps RBM15B, anticorps manf, anticorps manf.S

Sujet: MANF is a trophic factor for midbrain dopamine neuronsin vivo. It prevents the 6-OHDA-induced degeneration of dopamine neurons in rodent models of Parkinson’sdisease (Lindholm et al., 2008, Voutilainen et al., 2009). When administeredafter 6-OHDA-lesioning it restores the dopaminergic function and prevents degenerationof dopamine neurons in substantia nigra pars compacta

UniProt: P55145