V5 Epitope Tag anticorps

N° du produit ABIN962847

Cité dans 4 publications. Cet anticorps anti-V5 Epitope Tag Monoclonal Souris (Clone 4C12E11) (ABIN962847) détecte spécifiquement V5 Epitope Tag dans WB, ELISA, IP, IF, FACS et ICC.

Aperçu rapide pour V5 Epitope Tag anticorps (ABIN962847)

Antigène: V5 Epitope Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp V5 Epitope Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 4C12E11

Détail du produit anti-V5 Epitope Tag anticorps

Séquence: GKPIPNPLLG LDST

Specificité: THE^TM V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.

Attributs du produit: THE^TM V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.

Purification: Protein A affinity column

Immunogène: V5 tag peptide conjugated - KLH

Isotype: IgG2a

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/mL
Western blot: 0.2-1 µg/mL
Immunoprecipitation: 1-5 µg/mL
FACS: 1-3 µg for 1 x 10^6 cells
ICC/IF: 1-3 µg/mL
Fusion Partner Fusion of SP2/0-Ag14 myeloma and B-lymphocytes

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Références pour anticorps anti-V5 Epitope Tag (ABIN962847)

Liew, Assmann, Templin, Raum, Lipson, Rajan, Qiang, Hu, Kawamori, Lindberg, Philipson, Sonenberg, Goldfine, Stoffers, Mirmira, Urano, Kulkarni: "Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic ? cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 22, pp. E2319-28, (2014) (PubMed).

Wang, Li, Felsovalyi, Chen, Cardozo, Krogsgaard: "Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers." dans: ACS chemical biology, Vol. 9, Issue 9, pp. 2165-72, (2014) (PubMed).

Kim, Li, Ahn, Song, Zhang: "Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity." dans: Scientific reports, Vol. 4, pp. 7330, (2014) (PubMed).

Van Gorp, Van Breedam, Van Doorsselaere, Delputte, Nauwynck: "Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus." dans: Journal of virology, Vol. 84, Issue 6, pp. 3101-5, (2010) (PubMed).

Détails sur V5 Epitope Tag

Antigène: V5 Epitope Tag

Autre désignation: V5 Tag

Classe de substances: Tag

Sujet: The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.