AHSA1 anticorps

N° du produit ABIN964557

Détail du produit anti-AHSA1 anticorps

Antigène: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Reactivité: Humain, Souris, Chimpanzé

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp AHSA1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-G chromatography, followed by extensive dialysis against the buffer stated above. It is directed against human AHA1 protein.   A BLAST analysis was used to suggest cross-reactivity with AHA1 protein from mouse, human and chimpanzee based on 100% homology with the immunizing sequence.  Reactivity against homologues from other sources is not known.

Réactivité croisée: Humain, Chimpanzé

Attributs du produit: Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370-390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.

Purification: affinity purified

Stérilité: Sterile filtered

Immunogène: This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.
Immunogen Type: RecombinantProtein

Clone: 25F2-D9

Isotype: IgG2a kappa

Information d'application

Indications d'application: This Protein-G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 38-40 kDa in size corresponding to AHA1 protein by western blotting in the appropriate cell lysate or extract.

Commentaires:

Gene Name: Ahsa1

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Détails sur AHSA1

Antigène: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Autre désignation: AHA1 (AHSA1 Produits)

Synonymes: anticorps ahsa1, anticorps MGC52884, anticorps MGC132147, anticorps MGC80312, anticorps AHSA1, anticorps MGC89589, anticorps wu:fb83b05, anticorps zgc:136821, anticorps zgc:56075, anticorps AHA1, anticorps C14orf3, anticorps p38, anticorps BC023857, anticorps AHA1, activator of heat shock 90kDa protein ATPase homolog 1 S homeolog, anticorps activator of Hsp90 ATPase activity 1, anticorps AHA1, activator of heat shock 90kDa protein ATPase homolog 1 L homeolog, anticorps activator of HSP90 ATPase activity 1, anticorps AHA1, activator of heat shock 90kDa protein ATPase homolog 1, anticorps AHA1, activator of heat shock protein ATPase homolog 1a, anticorps AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast), anticorps AHA1, activator of heat shock protein ATPase 1, anticorps ahsa1.S, anticorps AHSA1, anticorps ahsa1.L, anticorps ahsa1, anticorps ahsa1a, anticorps Ahsa1

Sujet: Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo.  The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone.  Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle.  Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370–390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone.  Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER).  Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.
Synonyms: Aha1, Ahsa1 antibody, Activator of Hsp90 ATPase, Activator of 90 kDa heat shock protein ATPase homolog 1 antibody

ID gène: 217737

NCBI Accession: NP_666148

UniProt: Q8BK64