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Biotin anticorps

L’anticorps Lapin Polyclonal anti-Biotin a été validé pour WB, ELISA et IHC. Il convient pour détecter Biotin dans des échantillons de . Il y a 1 publication disponible.
Rockland
N° du produit ABIN964580
N° du produit (Fournisseur): 600-401-098

Aperçu rapide pour Biotin anticorps (ABIN964580)

Antigène

Biotin

Reactivité

Veuillez nous consulter SVP

Hôte

  • 64
  • 42
  • 38
Lapin

Clonalité

  • 83
  • 53
  • 1
Polyclonal

Conjugué

  • 37
  • 14
  • 9
  • 8
  • 8
  • 6
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Biotin est non-conjugé

Application

  • 78
  • 57
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  • 51
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  • 16
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  • 1
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • N° du produit (Fournisseur)

    600-401-098

    Fournisseur

    Rockland

    Fonction

    Biotin Antibody

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

    Attributs du produit

    Synonyms: rabbit anti Biotin Antibody, rabbit anti-biotin Antibody

    Purification

    Biotin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.

    Stérilité

    Sterile filtered

    Immunogène

    Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

    Immunogen Type: Other

    Isotype

    IgG
  • Indications d'application

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Biotin Antibody has been tested by ELISA and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

    Western Blot Dilution: 1:2,000 - 1:10,000

    ELISA Dilution: 1:200,000

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.02 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    This product is stable for several weeks at 4° C as an undiluted liquid. Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. 

    Date de péremption

    12 months
  • Goulielmaki, Ioannidou, Tsekrekou, Stratigi, Poutakidou, Gkirtzimanaki, Aivaliotis, Evangelou, Topalis, Altmüller, Gorgoulis, Chatzinikolaou, Garinis: "Tissue-infiltrating macrophages mediate an exosome-based metabolic reprogramming upon DNA damage." dans: Nature communications, Vol. 11, Issue 1, pp. 42, (2020) (PubMed).

  • Antigène

    Biotin

    Classe de substances

    Chemical

    Sujet

    Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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