Biotin anticorps (PE)
Aperçu rapide pour Biotin anticorps (PE) (ABIN964583)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
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N° du produit (Fournisseur)
- 600-408-098
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Fournisseur
- Rockland
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Fonction
- Biotin Antibody Phycoerythrin Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
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Attributs du produit
- Synonyms: rabbit anti Biotin Antibody Phycoerythrin Conjugated, rabbit anti-Biotin Antibody Phycoerythrin Conjugated
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Purification
- Rabbit Anti-Biotin was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
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Immunogène
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Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Immunogen Type: Other
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Isotype
- IgG
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Indications d'application
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Flow Cytometry Dilution: 1:100 - 1:250
Application Note: Anti-Biotin Phycoerythrin Conjugated Antibody has been tested by Western blot. The antibody may be used in amplification techniques, ELISA, immunoblotting, Immuno-histo-chemistry, in situ nucleic acid hybridization, flow cytometry or Immunofluorescence microscopy.
IF Microscopy Dilution: 1:100 - 1:250
Other: FLOW CYTOMETRY 1:100 - 1:250
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
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Date de péremption
- 12 months
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- Biotin
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Classe de substances
- Chemical
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Sujet
- Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Phycoerythrin Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
Antigène
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