Biotin anticorps (Rhodamine)

N° du produit ABIN964584

N° du produit (Fournisseur): 700-100-098

Cet anticorps anti-Biotin est un anticorps Chèvre Polyclonal détectant Biotin dans WB, FM et FLISA. Adapté pour .

Aperçu rapide pour Biotin anticorps (Rhodamine) (ABIN964584)

Antigène: Biotin

Fragment: F(ab')2 fragment

Reactivité: Veuillez nous consulter SVP

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Biotin est conjugé à/à la Rhodamine

Application: Western Blotting (WB), Fluorescence Microscopy (FM), FLISA

Détail du produit anti-Biotin anticorps (Rhodamine)

N° du produit (Fournisseur): 700-100-098

Fournisseur: Rockland

Fonction: F(Ab')2 Biotin Antibody Rhodamine Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Biotin conjugated IgG.

Attributs du produit: Synonyms: Goat F(ab')2 Anti-Biotin Rhodamine Conjugated Antibody, Goat F(ab')2 Anti-Biotin Antibody Rhodamine Conjugation, Goat Fab2 Anti-Biotin Antibody TRITC Conjugation

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogène:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin

Immunogen Type: Other

Isotype: IgG

Labeling Ratio: 2.9

Information d'application

Indications d'application:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Western Blot Dilution: User Optimized

FLISA Dilution: User Optimized

IF Microscopy Dilution: 1:500 - 1:2,500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Rhodamine Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.