Biotin anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN964587

N° du produit (Fournisseur): 700-105-098

Cet anticorps anti-Biotin Polyclonal Chèvre conjugué à Alkaline Phosphatase (AP) (ABIN964587) détecte spécifiquement Biotin dans WB, IHC et ELISA.

Aperçu rapide pour Biotin anticorps (Alkaline Phosphatase (AP)) (ABIN964587)

Antigène: Biotin

Fragment: F(ab')2 fragment

Reactivité: Veuillez nous consulter SVP

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Biotin est conjugé à/à la Alkaline Phosphatase (AP)

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-Biotin anticorps (Alkaline Phosphatase (AP))

N° du produit (Fournisseur): 700-105-098

Fournisseur: Rockland

Fonction: F(Ab')2 Biotin Antibody Alkaline Phosphatase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Biotinylated IgG.

Attributs du produit: Synonyms: Goat F(ab')2 Anti-Biotin alkaline phosphatase Conjugated Antibody, Goat F(ab')2 Anti-Biotin Antibody alkaline phosphatase Conjugation, Goat Fab2 Anti-Biotin Antibody alk phos Conjugation

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Other

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500-1:1,000

Application Note: This product has been assayed against 1.0 μg of Biotin conjugated IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product. 

Western Blot Dilution: 1:1,000-1:5,000

ELISA Dilution: 1:500 - 1:2,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet: Background: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers Background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.