CRASP-1 anticorps

N° du produit ABIN964610

N° du produit (Fournisseur): 200-401-c18

L’anticorps anti-CRASP-1 Polyclonal Lapin est utilisé pour la détection de CRASP-1 dans des échantillons de Borrelia burgdorferi. Il a été validé pour ELISA et WB.

Aperçu rapide pour CRASP-1 anticorps (ABIN964610)

Antigène: CRASP-1

Reactivité: Borrelia burgdorferi

Hôte: Lapin

Clonalité: Polyclonal

Application: ELISA, Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 200-401-c18

Fournisseur: Rockland

Fonction: CRASP-1 Antibody

Réactivité croisée (Details): This antibody is specific for Borrelia burgdorferi CRASP-1 protein.

Attributs du produit: Synonyms: rabbit anti-CRASP-1 Antibody, Borrelia burgdorferi CRASP-1, CRASP1, CRASP 1, Complement regulator acquiring protein 1

Purification: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.

Immunogène:

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi CRASP-1 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 26.9 kDa in size corresponding to Borrelia burgdorferi CRASP-1 protein by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: >1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: CRASP-1

Autre désignation: CRASP1

Sujet: Background: CRASP-1, or Complement Regulator-Acquiring Surface Protein 1, is a multifunctional protein of Lyme disease-causing B. burgdorferi that binds to several human extracellular matrix proteins and plasminogen, including factor H (resulting in inhibition of complement activation in mammals) and Human Bone Morphogenic Protein 2. These interactions may contribute to adhesion, bacterial colonization, and organ tropism and may allow dissemination of B. burgdorferi in the host. B. burgdorferi spirochetes express up to 5 complement regulator-acquiring surface proteins. Multiple copies of sequences analagous to CRASP-1 genes have been detected in Borrelia plasmids. Borrelia species contain a large number of plasmids, of linear and circular, some of which appear to repeat sequences or contain fragments of other genes. These regions may serve as potentially usable information for the survival of Borrelia in its multiple environments during its life cycle. In addition, the sequence for CRASP-1 contains a repeated sequence folded into a stable stem loop structure typical of RNA genes.

ID gène: 1194383

NCBI Accession: WP_010890397

UniProt: Q66ZC1