CTCF anticorps (C-Term)

N° du produit ABIN964615

N° du produit (Fournisseur): 600-401-c42

Cet anticorps Lapin Polyclonal détecte spécifiquement CTCF dans WB, IHC et ELISA. Il présente une réactivité envers Humain et Souris et a été mentionné dans 1 publication.

Aperçu rapide pour CTCF anticorps (C-Term) (ABIN964615)

Antigène: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CTCF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-CTCF anticorps

Épitope: C-Term

N° du produit (Fournisseur): 600-401-c42

Fournisseur: Rockland

Fonction: CTCF Antibody

Réactivité croisée (Details): This antibody reacts with endogenous CTCF protein.

Attributs du produit: Synonyms: rabbit anti-CTCF Antibody, Transcriptional repressor CTCF, 11-zinc finger protein, CCCTC-binding factor, CTCFL paralog

Purification: Anti-CTCF was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: CTCF affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: CTCF antibody has been tested by ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 82.8 kDa or 150 kDa in size corresponding to CTCF by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500-1:2:000

ELISA Dilution: 1:10,000-1:50,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.3 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store CTCF antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-CTCF (ABIN964615)

Majumder, Rupp, Yang-Iott, Koues, Kyle, Bassing, Oltz: "Domain-Specific and Stage-Intrinsic Changes in Tcrb Conformation during Thymocyte Development." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 3, pp. 1262-72, (2015) (PubMed).

Détails sur CTCF

Antigène: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Autre désignation: CTCF

Sujet: Background: Transcriptional repressor CTCF (also known as CCCTC-binding factor) is a transcription factor encoded by the CTCF gene. The CTCF, and the closely related Brother of the Regulator of Imprinted Sites (BORIS), are highly conserved zinc finger proteins implicated in diverse regulatory functions, including transcriptional activation/repression, insulation, imprinting, and X chromosome inactivation. Expression of BORIS is restricted to spermatocytes and is mutually exclusive of CTCF expression. CTCF is ubiquitously expressed in higher eukaryotes and contains a highly conserved and eleven zinc finger central DNA-binding domain, having very high homology between mouse, chicken, and human and is embedded within slightly divergent N and C termini. CTFC plays a critical role in the epigenetic regulation and chromatin remodeling. CTCF has been reported to bind to a variety of DNA target sites that perform distinct functions, including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting.

ID gène: 10664

NCBI Accession: NP_006556

UniProt: P49711