ErpD/Arp37 anticorps

N° du produit ABIN964635

N° du produit (Fournisseur): 200-401-c09

Cet anticorps anti-ErpD/Arp37 Polyclonal Lapin (ABIN964635) détecte spécifiquement ErpD/Arp37 dans ELISA et WB. L’anticorps est réactif avec des échantillons de Borrelia burgdorferi.

Aperçu rapide pour ErpD/Arp37 anticorps (ABIN964635)

Antigène: ErpD/Arp37

Reactivité: Borrelia burgdorferi

Hôte: Lapin

Clonalité: Polyclonal

Application: ELISA, Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 200-401-c09

Fournisseur: Rockland

Fonction: Erpd/Arp37 Antibody

Réactivité croisée (Details): It is directed against, and shows specific reactivity for, Borrelia burgdorferi ErpD protein.

Attributs du produit: Synonyms: Rabbit anti-ErpD Antibody, rabbit anti-Arp37 Antibody, rabbit anti-Arp37/ErpD Antibody, Arp37, Borrelia burgdorferi ErpD

Purification: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.

Immunogène:

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi ErpD/Arp37 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-ErpD antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at ~37.5 kDa and ~65 kDa in size corresponding to ErpD/Arp37 by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: >1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: ErpD/Arp37

Sujet: Background: This product is antibody made against ErpD (ospE/F-Related Protein D), also known as Arp37, from the spirochete Borrelia burgdorferi, which is carried by Ixodes ticks. Erp proteins from Borrelia burgdorferi are postulated to be lipoproteins, based on their predicted amino acid sequences. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. Several studies have demonstrated that infected humans and animals produce antibodies directed against Erp proteins within the first 2-4 weeks of infection, indicative of Erp synthesis during the initial stages of vertebrate infection. It is postulated that surface-exposed Erp proteins could facilitate interactions with host tissues during the establishment of vertebrate infection.

ID gène: 1194073

NCBI Accession: WP_010890266

UniProt: G5IXI1