GST anticorps (Alkaline Phosphatase (AP))
Aperçu rapide pour GST anticorps (Alkaline Phosphatase (AP)) (ABIN964665)
Antigène
Voir toutes GST AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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N° du produit (Fournisseur)
- 600-405-200
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Fournisseur
- Rockland
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Fonction
- GST Antibody Alkaline Phosphatase Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
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Attributs du produit
- Synonyms: rabbit anti-GST antibody alkaline phosphatase conjugation, alk phos conjugated rabbit anti-GST antibody, Glutathione-S-Transferase
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Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
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Stérilité
- Sterile filtered
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Immunogène
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Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.
Immunogen Type: Native Protein
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry Dilution: 1:200-1:1,000
Application Note: Anti-Glutathione-S-Transferase Alkaline Phosphatase Conjugate has been tested by ELISA and is suitable for western blotting and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:500-1:2,500
ELISA Dilution: 1:2,000-1:10,000
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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Date de péremption
- 12 months
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- GST (Glutathione S Transferase (GST))
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Autre désignation
- GST
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Sujet
- Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
Antigène
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