Tetanus Toxin C-Fragment (TTC) anticorps

N° du produit ABIN964673

N° du produit (Fournisseur): 100-401-894

L’anticorps Lapin Polyclonal anti- a été validé pour ELISA et WB. Il convient pour détecter dans des échantillons de Clostridium tetani.

Aperçu rapide pour Tetanus Toxin C-Fragment (TTC) anticorps (ABIN964673)

Antigène: Tetanus Toxin C-Fragment (TTC)

Reactivité: Clostridium tetani

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 100-401-894

Fournisseur: Rockland

Fonction: Tetanus Toxin Fragment C Antibody

Réactivité croisée (Details): This antibody is directed against the C fragment of Tetanus toxin derived from Clostridium tetani.

Attributs du produit: Synonyms: rabbit anti-Tetanus Toxin Fragment C Antibody , TTfC, tentoxylysin, tetX

Purification: The product was prepared from monospecific antiserum by delipidation and defibrination.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a polyhistidine-tagged TTFC fusion protein expressed in E. coli corresponding to full length TTFC.

Immunogen Type: Recombinant Protein

Information d'application

Indications d'application:

Application Note: This antibody has been tested for use in ELISA and western blot.  For western blots expect a band of approximately 52 kDa in size corresponding to full-length TTFC.  Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1,000 - 1:40,000

ELISA Dilution: 1:50,000 - 1:250,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 63 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: Tetanus Toxin C-Fragment (TTC)

Sujet: Background: Tetanus toxin C-fragment (TTc) is a non-toxic 52 kD polypeptide generated by papain cleavage of tetanus toxin, corresponding to the C-terminal 451 amino acids. The illness known as Tetanus is caused by a neurotoxin produced by the anaerobic bacterium Clostridium tetani. C. tetani is commonly found in soil, feces, and on many objects lying on the ground, such as rusty metal, hence puncture wounds are a common cause of tetanus. It acts upon the presynaptic membranes of both central and peripheral nervous systems to block the release of neurotransmitters.   In its toxic form tetanus neurotoxin is a 150 kDa protein, consisting of two major components: a light chain and heavy chain.  The light chain contains the enzymatic portion of the toxin and is responsible for its toxic effects.  The heavy chain binds to the neuron and aids delivery of the light chain to the neuron interior.   A single disulfide bond links light and heavy chains.

ID gène: 1061100

NCBI Accession: NP_783831

UniProt: P04958