NEDD1 anticorps

N° du produit ABIN964675

N° du produit (Fournisseur): 109-401-c38

L’anticorps Lapin Polyclonal anti-NEDD1 a été validé pour WB et ELISA. Il convient pour détecter NEDD1 dans des échantillons de Humain.

Aperçu rapide pour NEDD1 anticorps (ABIN964675)

Antigène: NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NEDD1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-NEDD1 anticorps

N° du produit (Fournisseur): 109-401-c38

Fournisseur: Rockland

Fonction: NEDD1 Antibody

Réactivité croisée (Details): This antibody reacts with endogenous Nedd1 protein.

Attributs du produit: Synonyms: GCP-WD, Neural precursor cell expressed developmentally down-regulated protein 1, rabbit anti-NEDD1 Antibody

Purification: This product was adsorbed against GST from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-NEDD1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the 343-667 region of human Nedd1.

Immunogen Type: Recombinant Protein

Information d'application

Indications d'application:

Application Note: This antiserum has been tested for use in ELISA and western blotting using a recombinant truncated Nedd1 protein. Specific conditions for reactivity and detection of Nedd1 should be optimized by the end user. Expect a band approximately ~73 kDa in size corresponding to Nedd1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:10,000

ELISA Dilution: 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 40 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur NEDD1

Antigène: NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))

Autre désignation: NEDD1

Sujet: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Microtubules are polymers of tubulin, which exist as heterodimers of alpha-tubulin and beta-tubulin. NEDD1 (neural precursor expressed, developmentally down-regulated protein1, also called GCP-WD) is a centrosomal protein that in mammals associates with the gamma-tubulin ring complex. Gamma-TuRC is critical for initiation, or nucleation, of the microtubule assembly. In association with this complex, NEDD1 plays an important role in targeting the gamma-TuRC complex to the site of microtubule nucleation and to the mitotic spindle. These events are essential for proper bipolar spindle formation and mitotic progression. Given the casual link between improper spindle function and tumorigenesis, characterization of Nedd1 function will be important to better understand various mechanisms underlying mitotic regulation, chromosome segregation, and cancer development.

ID gène: 121441

NCBI Accession: NP_001128647

Pathways: M Phase