Protein A anticorps

N° du produit ABIN964678

N° du produit (Fournisseur): 200-101-077

L’anticorps Chèvre Polyclonal anti-Protein A a été validé pour ELISA, WB et IP. Il convient pour détecter Protein A dans des échantillons de Staphylococcus aureus. Il y a 1 publication disponible.

Aperçu rapide pour Protein A anticorps (ABIN964678)

Antigène: Protein A

Reactivité: Staphylococcus aureus

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Protein A est non-conjugé

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-Protein A anticorps

N° du produit (Fournisseur): 200-101-077

Fournisseur: Rockland

Fonction: Protein A Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A  [Staphylococcus aureus] and anti-Goat Serum.

Attributs du produit: Synonyms: goat anti-Protein A Antibody, goat anti Protein A, goat anti PA

Purification: Protein A Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Protein A  [Staphylococcus aureus]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Protein A Antibody has been tested by western blot and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Optimal concentration in immunoassays should be determined by the researcher.

Western Blot Dilution: 1:2,000 - 1:10,000

Immunoprecipitation Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-Protein A (ABIN964678)

Treise, Huber, Klein-Rodewald, Heinemeyer, Grassmann, Basler, Adler, Rathkolb, Helming, Andres, Klaften, Landbrecht, Wieland, Strom, McCoy, Macpherson, Wolf, Groettrup, Ollert, Neff, Gailus-Durner et al.: "Defective immuno- and thymoproteasome assembly causes severe immunodeficiency. ..." dans: Scientific reports, Vol. 8, Issue 1, pp. 5975, (2018) (PubMed).

Détails sur Protein A

Antigène: Protein A

Sujet: Background: Anti-Protein A Antibody detects Protein A. Protein A is a 56 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus encoded by the spa gene. Protein A is an efficient binding protein to the immunoglobulins and is used in biochemical research because of its ability to bind. Protein A binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. It does not react with human IgG3 or IgD, nor will it react to mouse IgM, IgA or IgE. Protein A is commonly used in bioprocessing and bioseparations.