Luciferase anticorps

N° du produit ABIN964679

N° du produit (Fournisseur): 200-101-c44

L’anticorps Chèvre Polyclonal anti-Luciferase a été validé pour WB, ELISA, IHC et FM. Il convient pour détecter Luciferase dans des échantillons de Photinus pyralis. Il y a 2+ publications disponibles.

Aperçu rapide pour Luciferase anticorps (ABIN964679)

Antigène: Luciferase

Reactivité: Photinus pyralis

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Luciferase est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Détail du produit anti-Luciferase anticorps

N° du produit (Fournisseur): 200-101-c44

Fournisseur: Rockland

Fonction: Luciferase Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)].

Attributs du produit: Synonyms: goat anti-Luciferase Antibody, Luciferin 4-monooxygenase, Photinus pyralis (Common eastern firefly) (Lampyris pyralis)

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Luciferase [Photinus pyralis (Firefly)]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1500

Application Note: Anti-Luciferase Antibody has been tested in Western Blot, IHC, IF, and ELISA. Expect a band ~60 kDa in appropriate cell lysates. Although not tested, this antibody would be useful in immunoprecipitation, immunocytochemistry, and most immunological methods requiring high titers and specificity.

Western Blot Dilution: 1:1000 - 1:5000

ELISA Dilution: 1:200 - 1:1000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-Luciferase (ABIN964679)

Pommier, Anaparthy, Memos, Kelley, Gouronnec, Yan, Auffray, Albrengues, Egeblad, Iacobuzio-Donahue, Lyons, Fearon: "Unresolved endoplasmic reticulum stress engenders immune-resistant, latent pancreatic cancer metastases." dans: Science (New York, N.Y.), Vol. 360, Issue 6394, (2018) (PubMed).

Kenny, Duval, Sammut, Steele, Pritchard, Atherton, Argent, Dimaline, Dockray, Varro: "Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 3, pp. G431-41, (2008) (PubMed).

Détails sur Luciferase

Antigène: Luciferase

Sujet: Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.