Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (C-Term) anticorps

Détails pour le produit réf. ABIN964697
Antigène
  • FSGS2
  • TRP6
  • AV025995
  • LLHWJM002
  • LLHWJM003
  • LLHWJM004
  • TRP-6
  • Trrp6
  • mtrp6
  • trp6
  • bZ1P14.9
  • si:rp71-1p14.9
  • trpc6
  • transient receptor potential cation channel subfamily C member 6
  • transient receptor potential cation channel, subfamily C, member 6
  • transient receptor potential cation channel, subfamily C, member 6a
  • TRPC6
  • Trpc6
  • trpc6a
  • trpc6
Épitope
C-Term
37
21
6
3
2
2
2
1
1
1
1
1
1
1
Reactivité
Humain, Chimpanzé
100
60
35
7
5
4
4
3
2
2
1
1
Hôte
Souris
79
42
5
1
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
4
4
4
4
4
4
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
105
60
36
13
12
5
3
2
2
2
1
Options
Immunogène This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.
Immunogen Type: Peptide
Clone 3F2-H10-F2
Isotype IgG1 kappa
Specificité This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human TRPC6 protein. A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.
 Réactivité croisée Chimpanzé
Attributs du produit TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Stérilité Sterile filtered
Antigène
Autre désignation TRPC6 (TRPC6 Antibody Extrait)
Sujet TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Synonyms: TRPC6, TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6
ID gène 7225, 5730102
UniProt Q9Y210
Indications d'application This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Expect a band approximately 106 kDa in size corresponding to TRPC6 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Strong staining was observed in adrenal, Purkinje neurons, cortical neurons, heart, ganglion cells, renal tubules, Sertoli cells, hepatocytes, skeletal muscle, exocrine pancreas, and germinal centers of lymphoid follicles. Moderate staining was observed in colon epithelium and plasma cells, B-lymphocytes, and parafollicular cells of the thyroid. Faint staining was seen in respiratory epithelium. Prostate and placenta were negative for staining. The antibody produced minimal to no background staining and appeared very specific at 2.5 µg/mL.
Commentaires

Gene Name: TRPC6

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C/-20 °C
Stockage commentaire Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Date de péremption 12 months
Images (Fournisseur)
Immunohistochemistry (IHC) image for anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (C-Term) antibody (ABIN964697) Immunohistochemistry using anti-TRPC6 monoclonal antibody shows detection of TRPC6 i...
Western Blotting (WB) image for anti-Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (C-Term) antibody (ABIN964697) Western Blot of Mouse anti-TRPC6 Antibody Lane 1: Mouse Kidney WCL Load: 10 µg per la...
Produit citée dans: Alonso-González, Cabo, San José, Gago, Suazo, García-Suárez, Cobo, Vega: "Human Digital Meissner Corpuscles Display Immunoreactivity for the Multifunctional Ion Channels Trpc6 and Trpv4." dans: Anatomical record (Hoboken, N.J. : 2007), Vol. 300, Issue 6, pp. 1022-1031, 2018 (PubMed).

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