TRPC6 anticorps (C-Term)

N° du produit ABIN964697

Détail du produit anti-TRPC6 anticorps

Antigène: TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))

Épitope: C-Term

Reactivité: Humain, Chimpanzé

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TRPC6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificité: This product was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human TRPC6 protein. A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.

Réactivité croisée: Chimpanzé

Attributs du produit: TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.

Stérilité: Sterile filtered

Immunogène: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.
Immunogen Type: Peptide

Clone: 3F2-H10-F2

Isotype: IgG1 kappa

Information d'application

Indications d'application: This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Expect a band approximately 106 kDa in size corresponding to TRPC6 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Strong staining was observed in adrenal, Purkinje neurons, cortical neurons, heart, ganglion cells, renal tubules, Sertoli cells, hepatocytes, skeletal muscle, exocrine pancreas, and germinal centers of lymphoid follicles. Moderate staining was observed in colon epithelium and plasma cells, B-lymphocytes, and parafollicular cells of the thyroid. Faint staining was seen in respiratory epithelium. Prostate and placenta were negative for staining. The antibody produced minimal to no background staining and appeared very specific at 2.5 µg/mL.

Commentaires:

Gene Name: TRPC6

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Références pour anticorps anti-TRPC6 (ABIN964697)

Alonso-González, Cabo, San José, Gago, Suazo, García-Suárez, Cobo, Vega: "Human Digital Meissner Corpuscles Display Immunoreactivity for the Multifunctional Ion Channels Trpc6 and Trpv4." dans: Anatomical record (Hoboken, N.J. : 2007), Vol. 300, Issue 6, pp. 1022-1031, (2018) (PubMed).

Détails sur TRPC6

Antigène: TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))

Autre désignation: TRPC6 (TRPC6 Produits)

Synonymes: anticorps FSGS2, anticorps TRP6, anticorps AV025995, anticorps LLHWJM002, anticorps LLHWJM003, anticorps LLHWJM004, anticorps TRP-6, anticorps Trrp6, anticorps mtrp6, anticorps trp6, anticorps bZ1P14.9, anticorps si:rp71-1p14.9, anticorps trpc6, anticorps transient receptor potential cation channel subfamily C member 6, anticorps transient receptor potential cation channel, subfamily C, member 6, anticorps transient receptor potential cation channel, subfamily C, member 6a, anticorps TRPC6, anticorps Trpc6, anticorps trpc6a, anticorps trpc6

Sujet: TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Synonyms: TRPC6, TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6

ID gène: 7225, 5730102

UniProt: Q9Y210