Streptavidin anticorps

N° du produit ABIN964708

N° du produit (Fournisseur): 200-401-095

Cet anticorps Lapin Polyclonal détecte spécifiquement Streptavidin dans ELISA, WB, IHC et DB. Il présente une réactivité envers Streptomyces avidinii.

Aperçu rapide pour Streptavidin anticorps (ABIN964708)

Antigène: Streptavidin

Reactivité: Streptomyces avidinii

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Streptavidin est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Détail du produit anti-Streptavidin anticorps

N° du produit (Fournisseur): 200-401-095

Fournisseur: Rockland

Fonction: Streptavidin Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii].

Attributs du produit: Synonyms: rabbit anti-Streptavidin Antibody, rabbit anti Streptavidin

Purification: Anti-Streptavidin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Streptavidin [Streptomyces avidinii]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,500 - 1:5,000

Application Note: Anti-Streptavidin antibody has been tested by ELISA and dot blot and is suitable for western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:3,000 - 1:12,000

ELISA Dilution: 1:200,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Streptavidin

Antigène: Streptavidin

Sujet: Background: Streptavidin antibody is a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7). With a dissociation constant (Kd) on the order of ~10-14 Mol/L,[1] the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is used extensively in molecular biology and bio-nanotechnology due to the streptavidin-biotin complex's resistance to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH . Anti-STREPTAVIDIN antibody is ideal for investigators involved in GFP and Epitope research.