OspA anticorps

N° du produit ABIN964719

N° du produit (Fournisseur): 200-401-c13

Cet anticorps anti-OspA est un anticorps Lapin Polyclonal détectant OspA dans ELISA et WB. Adapté pour Borrelia burgdorferi.

Aperçu rapide pour OspA anticorps (ABIN964719)

Antigène: OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))

Reactivité: Borrelia burgdorferi

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp OspA est non-conjugé

Application: ELISA, Western Blotting (WB)

Détail du produit anti-OspA anticorps

N° du produit (Fournisseur): 200-401-c13

Fournisseur: Rockland

Fonction: OspA Antibody

Réactivité croisée (Details): This antibody is specific for Borrelia burgdorferi OspA protein.

Attributs du produit: Synonyms: rabbit anti-OspA Antibody, Outer surface protein A, Borrelia burgdorferi OspA, ospA, BB_A15, Plasmid lp54

Purification: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.

Immunogène:

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspA protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 28.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: >1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur OspA

Antigène: OspA (Borrelia Burgdorferi Outer Surface Protein A (OspA))

Autre désignation: ospA

Classe de substances: Bacteria Protein

Sujet: Background: Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression.

ID gène: 1194357

NCBI Accession: NP_045688

UniProt: P0CL66