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OspB anticorps

Reactivité: Borrelia burgdorferi WB Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN964721
  • Antigène
    OspB
    Reactivité
    Borrelia burgdorferi
    Hôte
    Lapin
    Clonalité
    Polyclonal
    Application
    Western Blotting (WB)
    Specificité
    This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspB protein. A BLAST analysis was used to suggest cross-reactivity with OspB from B. burgdorferi, afzelii, spielmanii, and garinii sources based on 100% homology with the immunizing sequence, and with B. valaisiana based on 99% homology. Cross-reactivity with OspB from other sources has not been determined.
    Attributs du produit
    OspB, is one of the major Outer Surface Proteins of the outer membrane of Borrelia burgdorferi, which is composed of various unique outer surface proteins (Osp) that have been characterized (OspA through OspF). The Osp proteins are lipoproteins anchored by N-terminally attached fatty acid molecules to the membrane. They are presumed to play a role in virulence, transmission, or survival in the tick. Two of the major surface Ag of Borrelia burgdorferi, the 31-kDa OspA and 34-kDa OspB proteins, show a high degree of sequence similarity, are encoded by a 49-kb plasmid and share a common promoter, and are coordinately transcribed. OspA, OspB, and OspD are expressed by B. burgdorferi residing in the gut of unfed ticks, suggesting that they promote the persistence of the spirochete in ticks between blood meals. OspB has a contributing role in the adherence of B. burgdorferi to the tick gut. B. burgdorferi has the ability to vary its surface proteins in response to immune attack.
    Purification
    purified
    Stérilité
    Sterile filtered
    Immunogène
    MBP-fusion protein corresponding to Borrelia burgdorferi OspB protein.
    Immunogen Type: RecombinantProtein
    Isotype
    IgG
  • Indications d'application
    Suggested dilutions:
    ELISA : 1:13,000
    Western Blot : 1:1,000
    Other Dilution: User Optimized
    Commentaires

    Gene Name: ospB, locus BB_A16

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.1 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Stock
    -20 °C
    Stockage commentaire
    Store vial at -20° C or below prior to opening. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Date de péremption
    3 months
  • Neelakanta, Li, Pal, Liu, Beck, DePonte, Fish, Kantor, Fikrig: "Outer surface protein B is critical for Borrelia burgdorferi adherence and survival within Ixodes ticks." dans: PLoS pathogens, Vol. 3, Issue 3, pp. e33, (2007) (PubMed).

    Liang, Caimano, Radolf, Fikrig: "Borrelia burgdorferi outer surface protein (osp) B expression independent of ospA." dans: Microbial pathogenesis, Vol. 37, Issue 1, pp. 35-40, (2004) (PubMed).

    Haake: "Spirochaetal lipoproteins and pathogenesis." dans: Microbiology (Reading, England), Vol. 146 ( Pt 7), pp. 1491-504, (2000) (PubMed).

  • Antigène
    OspB
    Sujet
    OspB, is one of the major Outer Surface Proteins of the outer membrane of Borrelia burgdorferi, which is composed of various unique outer surface proteins (Osp) that have been characterized (OspA through OspF). The Osp proteins are lipoproteins anchored by N-terminally attached fatty acid molecules to the membrane. They are presumed to play a role in virulence, transmission, or survival in the tick. Two of the major surface Ag of Borrelia burgdorferi, the 31-kDa OspA and 34-kDa OspB proteins, show a high degree of sequence similarity, are encoded by a 49-kb plasmid and share a common promoter, and are coordinately transcribed. OspA, OspB, and OspD are expressed by B. burgdorferi residing in the gut of unfed ticks, suggesting that they promote the persistence of the spirochete in ticks between blood meals. OspB has a contributing role in the adherence of B. burgdorferi to the tick gut. B. burgdorferi has the ability to vary its surface proteins in response to immune attack.
    Synonyms: Outer surface protein B, Borrelia burgdorferi OspB
    ID gène
    1194340
    UniProt
    P17739
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