p39 anticorps

N° du produit ABIN964722

N° du produit (Fournisseur): 200-401-c17

Cet anticorps anti- est un anticorps Lapin Polyclonal détectant dans WB et ELISA. Adapté pour Borrelia burgdorferi et Borrelia afzelii. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour p39 anticorps (ABIN964722)

Antigène: p39

Reactivité: Borrelia burgdorferi, Borrelia afzelii

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA

Détail du produit

N° du produit (Fournisseur): 200-401-c17

Fournisseur: Rockland

Fonction: p39 Antibody

Réactivité croisée (Details): This antibody is specific for Borrelia burgdorferi p39 protein.

Attributs du produit: Synonyms: rabbit anti-p39 Antibody, Basic membrane protein A, Borrelia burgdorferi bmpA, immunodominant antigen P39, membrane lipoprotein BmpA

Purification: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.

Immunogène:

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi p39 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 35.4 kDa in size corresponding to Borrelia burgdorferi p39 protein by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: >1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Karvonen, Tammisto, Nykky, Gilbert: "Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells." dans: Microorganisms, Vol. 10, Issue 2, (2022) (PubMed).

Détail du antigène

Antigène: p39

Sujet: Background: The p39 protein, or Basic membrane protein A, is one of the immunogenic cell membrane components of Borrelia burgdorferi, the spirochete carried by Ixodes ticks. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. BmpA is expressed during the invasion of the spirochete and in the evolution of the arthritis of Lyme disease in mammals. It belongs to the BMP lipoprotein family. The major products of the B. burgdorferi basic membrane protein (bmp) A/B operon that are induced in murine and human joints possess inflammatory properties. Non-lipidated and lipidated versions of BmpA have been shown to induce the pro-inflammatory cytokine TNF-α and IL-1ß in human synovial cells. The induction of cytokine responses in synovial cells via activation of the NF-kappaB and p38 MAP kinase pathways could potentially contribute to the genesis of Lyme arthritis. The BmpA outer-surface protein is an important antigen for serodiagnosis of human infection. B. burgdorferi adheres to host extracellular matrix components, including laminin, but may not bind mammalian type I or type IV collagens or fibronectin.

ID gène: 1195220

NCBI Accession: WP_002656850

UniProt: Q45010