Myeloperoxidase anticorps

N° du produit ABIN964728

N° du produit (Fournisseur): 200-4181

Cet anticorps anti-Myeloperoxidase est un anticorps Lapin Polyclonal détectant Myeloperoxidase dans WB, ELISA et IP. Adapté pour Humain.

Aperçu rapide pour Myeloperoxidase anticorps (ABIN964728)

Antigène: Myeloperoxidase (MPO)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Myeloperoxidase est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-Myeloperoxidase anticorps

N° du produit (Fournisseur): 200-4181

Fournisseur: Rockland

Fonction: Myeloperoxidase Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Myeloperoxidase [Human Leukocytes].

Attributs du produit: Synonyms: rabbit anti-Myeloperoxidase Antibody, rabbit anti-MPO antibody

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Myeloperoxidase [Human Leukocytes]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This antibody is tested by western blotting suitable for ELISA and immunoprecipitation.  Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods.  Anti-Human Myeloperoxidase may react with MPO from other sources. Anti-Human Myeloperoxidase detects neutrophilic granulocytes and monocytes in blood and precursors of granulocytes in the bone marrow. This antibody may also detect myeloid leukemias of the bone marrow as well as other sites.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Myeloperoxidase

Antigène: Myeloperoxidase (MPO)

Autre désignation: MPO

Sujet: Background: Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa).  Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle.  Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE.  Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform.  The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5 % . MPO is inhibited by azide and other compounds.  MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant.  HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H₂O₂).  In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.

ID gène: 4353

UniProt: P05164

Pathways: Chromatin Binding