SAE1 anticorps (Internal Region)

N° du produit ABIN964924

N° du produit (Fournisseur): 600-401-c51

Cet anticorps Lapin Polyclonal détecte spécifiquement SAE1 dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour SAE1 anticorps (Internal Region) (ABIN964924)

Antigène: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SAE1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-SAE1 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-c51

Fournisseur: Rockland

Fonction: SAE1 Antibody

Réactivité croisée (Details): This purified antibody is directed against human SUMO Activating Enzyme E1 protein.

Attributs du produit: Synonyms: rabbit anti-SAE1 antibody, rabbit anti-SUMO activating enzyme subunit 1 antibody, Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, SUA1, SAE-1

Purification: The product was purified from monospecific antiserum by affinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human SUMO Activating Enzyme E1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~37 kDa in size corresponding to SAE1 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 2 μg/mL

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur SAE1

Antigène: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Autre désignation: SAE1

Sujet: Background: SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.

ID gène: 10055, 42559897

UniProt: Q9UBE0