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CD13 Myeloid Cell anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement dans FACS, IHC (af), IHC (fro) et IF. Il présente une réactivité envers Humain et a été mentionné dans 3+ publications.
N° du produit ABIN965764

Aperçu rapide pour CD13 Myeloid Cell anticorps (ABIN965764)

Antigène

CD13 Myeloid Cell

Reactivité

Humain

Hôte

  • 1
Souris

Clonalité

  • 1
Monoclonal

Conjugué

  • 1
Inconjugué

Application

Flow Cytometry (FACS), Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Clone

B347 (B-F10)
  • Specificité

    This antibody reacts with CD13, a 150 kD cell surface glycoprotein. The CD13 antigen is identical to aminopeptidase N. The specificity of these antibodies are similar to those of WM-15 and My7.

    Purification

    Purified.

    Immunogène

    Human KG-1 (myeloid) cultured cells.

    Isotype

    IgG1 kappa
  • Indications d'application

    Immunofluorescence: 5-20 ug/mL.
    Flow cytometry: 1-5 ug/10^6 cells.
    Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
    Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections.

    Commentaires

    Recommended Positive Control: Tonsil and Lymph Node

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.4 mg/ml.

    Buffer

    50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C
  • Look, Ashmun, Shapiro, Peiper: "Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N." dans: The Journal of clinical investigation, Vol. 83, Issue 4, pp. 1299-307, (1989) (PubMed).

    Sakai, Hattori, Sagawa, Yokoyama, Takatsuki: "Biochemical and functional characterization of MCS-2 antigen (CD13) on myeloid leukemic cells and polymorphonuclear leukocytes." dans: Cancer research, Vol. 47, Issue 21, pp. 5572-6, (1987) (PubMed).

    Look, Peiper, Rebentisch, Ashmun, Roussel, Rettenmier, Sherr: "Transfer and expression of the gene encoding a human myeloid membrane antigen (gp150)." dans: The Journal of clinical investigation, Vol. 75, Issue 2, pp. 569-79, (1985) (PubMed).

  • Antigène

    CD13 Myeloid Cell

    Autre désignation

    CD13,Myeloid Cell

    ID gène

    290
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