CD20 (B Cells) (Extracellular Domain) anticorps

N° du produit ABIN965777

L’anticorps Souris Monoclonal anti- a été validé pour FACS, IF et IHC (fro). Il convient pour détecter dans des échantillons de Humain. Il y a 2+ publications disponibles.

Aperçu rapide pour CD20 (B Cells) (Extracellular Domain) anticorps (ABIN965777)

Antigène: CD20 (B Cells)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B342 (B9E9)

Détail du produit

Épitope: Extracellular Domain

Specificité: This antibody reacts with an antigen present on most of the B-cells. They react with a 33 kD polypeptide and a minor component of a 30 kD polypeptide present in B-cells.

Purification: Purified.

Immunogène: Lymphoblastoid cell line DAUDI.

Isotype: IgG2a

Information d'application

Indications d'application: Immunofluorescence: 5-20 ug/mL
Flow cytometry: 1-5 ug/10^6 cells
Western blotting: 1-5 ug/mL.
Immunohistochemistry: 5-20 mg/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections.

Commentaires:

Cellular Localization: Cell membrane.
Recommended Positive Control: Tonsil and Lymph Node

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/ml.

Buffer: 50 mM Sodium phosphate, 150 mM Sodium Chloride, BSA 0.2%, pH 8.0

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Référence

Kanzaki, Shibata, Mogami, Kojima: "Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells." dans: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13099-104, (1995) (PubMed).

Bubien, Zhou, Bell, Frizzell, Tedder: "Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes." dans: The Journal of cell biology, Vol. 121, Issue 5, pp. 1121-32, (1993) (PubMed).

Détail du antigène

Antigène: CD20 (B Cells)

Poids moléculaire: 33-37 kDa

ID gène: 931