CD56 anticorps

N° du produit ABIN7446175

Cet anticorps Souris Monoclonal détecte spécifiquement CD56 dans FACS et IHC (fro). Il présente une réactivité envers Humain.

Aperçu rapide pour CD56 anticorps (ABIN7446175)

Antigène: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD56 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B332(123A8)

Détail du produit anti-CD56 anticorps

Specificité: This antibody reacts with a 135/220 kD glycosylated and sialyated protein, expressed on CD2+, CD3-, CD16+ natural killer cells and neuroectodermal cells.

Purification: Purified.

Immunogène: Membrane preparation from small cell lung carcinoma.

Isotype: IgG1

Information d'application

Indications d'application: Immunofluorescence: 10-20 micrograms/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Westernblotting: 1-5 ug/mL.
Immunohistochemistry: 4 -5 micrograms/mL on frozen and formalin fixed tissue sections using avidin-biotin system. The formalin fixed tissue requires boiling in 10 mM citrate buffer, pH 6.0 for 10-20 mins. This antibody is used for identification of NK- cells.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections. This antibody can be used with formalin-fixed paraffin-embedded tissue sections. These tissue require boiling in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling at RT. The antibody may be used at a dilution of 2-4 micrograms/mL in IHC.

Commentaires:

Recommended Positive Control: Human retinoblastoma or neuroblastoma, small Granulocytes Lung Carcinoma or wilms tumors.

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/ml.

Buffer: 10 mM Sodium phosphate, 150 mM Sodium Chloride, 0.2% BSA, pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur CD56

Antigène: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Autre désignation: CD56,Natural Killer Cell

Poids moléculaire: 125, 145, 180 kDa.

ID gène: 4684